| 2000 AF2 season | |
|---|---|
| League | AF2 |
| Sport | Arena football |
| Regular season | |
| Season champions | Quad City Steamwheelers |
| Playoffs | |
| Semifinals champions | Quad City Steamwheelers |
| Semifinals runners-up | Norfolk Nighthawks |
| Semifinals champions | Tennessee Valley Vipers |
| Semifinals runners-up | Augusta Stallions |
| ArenaCup I | |
| Champions | Quad City Steamwheelers |
| Runners-up | Tennessee Valley Vipers |
The 2000 AF2 season was the first season of the af2. It was succeeded by 2001. The league champions were the Quad City Steamwheelers, who capped off a perfect season with a victory over the Tennessee Valley Vipers in ArenaCup I. This is the only AF2 season lacking division alignment.