Back مجهر إلكتروني فائق البرودة Arabic Kryoelektronová mikroskopie Czech Kryoelektronenmikroskopie German Criomicroscopía electrónica Spanish میکروسکوپ الکترونی کرایو Persian Kryoelektronimikroskopia Finnish Cryomicroscopie électronique French Microscopia electrónica crioxénica Galician מיקרוסקופ אלקטרונים קריוגני HE Microscopia crioelettronica Italian

Cryogenic electron microscopy

Titan Krios at the University of Leeds

Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane.[1] While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.[2] This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.[3]

In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution."[4] Nature Methods also named cryo-EM as the "Method of the Year" in 2015.[5]

  1. ^ Tivol WF, Briegel A, Jensen GJ (October 2008). "An improved cryogen for plunge freezing". Microscopy and Microanalysis. 14 (5): 375–379. Bibcode:2008MiMic..14..375T. doi:10.1017/S1431927608080781. PMC 3058946. PMID 18793481.
  2. ^ Cheng Y, Grigorieff N, Penczek PA, Walz T (April 2015). "A primer to single-particle cryo-electron microscopy". Cell. 161 (3): 438–449. doi:10.1016/j.cell.2015.03.050. PMC 4409659. PMID 25910204.
  3. ^ Stoddart C (1 March 2022). "Structural biology: How proteins got their close-up". Knowable Magazine. doi:10.1146/knowable-022822-1. S2CID 247206999. Retrieved 25 March 2022.
  4. ^ Cite error: The named reference Cressey 2017 was invoked but never defined (see the help page).
  5. ^ Doerr A (January 2017). "Cryo-electron tomography". Nature Methods. 14 (1): 34. doi:10.1038/nmeth.4115. ISSN 1548-7091. S2CID 27162203.

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