Label-free quantification

Label-free quantification is a method in mass spectrometry that aims to determine the relative amount of proteins in two or more biological samples. Unlike other methods for protein quantification, label-free quantification does not use a stable isotope containing compound to chemically bind to and thus label the protein.[1][2]

  1. ^ Bantscheff M, Schirle M, Sweetman G, Rick J, Kuster B (October 2007). "Quantitative mass spectrometry in proteomics: a critical review". Analytical and Bioanalytical Chemistry. 389 (4): 1017–31. doi:10.1007/s00216-007-1486-6. PMID 17668192.
  2. ^ Asara JM, Christofk HR, Freimark LM, Cantley LC (March 2008). "A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen". Proteomics. 8 (5): 994–9. doi:10.1002/pmic.200700426. PMID 18324724.

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